Structural Variation Analysis of Mutated Nannochloropsis oceanica Caused by Zeocin Through Genome Re-Sequencing     

LIN Genmei  ZHANG Zhongyi  GUO Li  DING Haiyan  YANG Guanpin 《中国海洋大学学报(英文版)》2018,(5)

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BDS/GPS/GLONASS在密林地区组合定位性能分析          下载免费PDF全文

郭现伟  姬生月  王振杰  鲁洋为 《海洋测绘》2018,(5):55-58

随着北斗卫星导航系统的建成与运行,目前已形成多系统导航定位的局面。基于短基线,首先对密林地区的GPS、BDS、GLONASS的卫星可见性以及伪距观测值的质量进行了简单的评价,然后分析了密林地区GPS、BDS、GLONASS单系统、双系统及三系统组合模式下单点定位及伪距差分定位性能。结果表明:在密林地区,GNSS多系统组合能够明显提高定位精度,GPS/BDS组合定位的精度优于GPS/GLONASS、BDS/GLONASS组合,而GPS/BDS/GLONASS的定位精度最高。  相似文献   

Effect of sand on the vacuum consolidation of dredged slurry     

Jun Wang  Yuanqiang Cai  Xueyu Geng  Fengqi Xu 《Marine Georesources & Geotechnology》2018,36(2):238-244

Vacuum preloading is often used to improve the geotechnical properties of dredged slurry. Although the performance of this method has improved with rapidly developing technology, soil columns usually formed on the drainage boundary induce the decrease of permeability around the boundary, thereby limiting the further development of this method. To address this issue, this paper proposes a method for pretreating the slurry combined with sand prior to vacuum consolidation. This method partially replaces the fine particles with sand to reduce the formation of soil columns. Two groups of vacuum preloading tests were performed to investigate the effect of sand content and sand grain size on the vacuum consolidation of dredged slurry. The test results revealed that for a given sand grain size, increasing the sand content of the sand–slurry mixture increased the pore water drainage and accelerated the dissipation of pore water pressure, thereby increasing the vane shear strength. In contrast, for a constant sand content, the samples containing coarse sand exhibited increased pore water drainage and accelerated dissipation of pore water pressure, thereby increasing the vane shear strength of the soil.  相似文献   

栉孔扇贝foxl2基因在卵子发生中的必要性     

刘晓玲  刘军  王振东  王振华  李彦伸  崔龙波 《海洋科学》2018,42(4):127-131

采用RNAi技术研究了栉孔扇贝(Chlamysfarreri)foxl2基因在卵子发生和卵巢功能维持中的作用。使用体外合成的栉孔扇贝foxl2双链RNA(dsRNA),以每只50μg/次的剂量注射进闭壳肌中,连续注射2次(第一次注射后7天,再进行第二次注射);以注射相同体积PBS(相同注射方法)的扇贝作为阴性对照组,以不注射扇贝作为空白对照组。qRT-PCR检测干扰组扇贝卵巢中的foxl2 mRNA表达量较空白对照组下降了62%, Western blotting检测该蛋白在卵巢中的含量也明显下降,显示靶基因的表达水平被有效地敲降。组织学观察发现,栉孔扇贝foxl2干扰后的卵巢中卵母细胞形态异常、细胞核固缩,卵子发生明显受阻。表明该基因在栉孔扇贝卵子发生中起重要作用。  相似文献   

基于OEM板的GNSS原始数据解码与质量分析     

郭亮亮  李建文  魏绒绒  代桃高  刘瑞宁 《大地测量与地球动力学》2018,38(1):106-110

介绍Trimble BD970 OEM板接收BINEX格式数据流的结构和解码原理，得到相应的多系统观测文件和星历文件，成功完成对实测数据的解码。介绍GNSS观测数据质量评估的指标及方法，并基于解码后的数据对BD970 OEM板进行数据分析，得到该板卡的观测数据质量相关指标值，为后续的产品应用提供可靠保障。  相似文献   

两种方法提取肠浒苔挥发油成分的比较研究     

郭嘉颖  陈思羽  方倩云  苏培  冯丹青  柯才焕 《应用海洋学学报》2018,37(4):585-593

本研究分别采用水蒸气蒸馏法(SD)和溶剂提取法(SE)提取肠浒苔(Ulva intestinalis)的挥发油,通过GC-MS技术对其进行化学成分分析,以峰面积归一法对各成分进行相对定量.结果显示,SD法对肠浒苔挥发油的提取率(0. 038%)低于SE法(0. 800%). SD法所获得的肠浒苔挥发油共鉴定出46种成分,占挥发油总成分的81. 63%,SE法所获得的挥发油共鉴定出30种成分,占挥发油总成分的94. 89%,两者相同成分21种,主要成分均为烃类和醛类物质. SD样品中十五醛的相对含量最高(18. 12%),SE样品中8-十八烷烯的相对含量最高(51. 49%).  相似文献   

圆形数值波浪水池斜向和多向入射波浪模拟研究     

任兴月  陶军  彭伟 《海洋工程》2018,36(4):78-87

为了研究斜向入射波浪,基于三维不可压缩两相流模型,开发了一套圆形数值波浪水池数值模型。在圆形波浪水池中,通过源项造波法成功生成了任意入射方向的波浪,并且利用人工摩擦项模拟阻尼区以数值耗散反射波浪。模型基于嵌入式多块网格体系,采用FVM法(finite volume method)离散Navier-Stokes方程,VOF法(volume of fluid)追踪自由水面。试验结果表明,斜向入射波浪的模拟结果与理论值基本一致,圆形波浪水池在模拟斜向入射波浪时,有效区域的面积较传统波浪水池显著增大,而且有效区域受波浪入射角度的影响也较小。同时,通过叠加多列斜向入射波浪,模拟出了多向交叉波列,并通过与理论结果对比,发现其具有较高的精度。  相似文献   

南海冬季之西边界流：1998年2月的水文结构与体积输运     

李立  郭小刚  吴日升 《海洋学报(英文版)》2018,37(3):1-7

The unique survey in December 1998 mapped the entire western boundary area of the South China Sea(SCS),which reveals the three-dimensional structure and huge volume transport of the swift and narrow winter western boundary current of the SCS(SCSwwbc) in full scale. The current is found to flow all the way from the shelf edge off Hong Kong to the Sunda Shelf with a width around 100 km and a vertical scale of about 400 m. It appears to be the strongest off the Indo-China Peninsula, where its volume transport reached over 20×10~6 m~3/s. The current is weaker upstream in the northern SCS to the west of Hong Kong. A Kuroshio loop or detached eddy intruded through the Luzon Strait is observed farther east where the SCSwwbc no more exists. The results suggest that during the survey the SCSwwbc was fed primarily by the interior recirculation of the SCS rather than by the"branching" of the Kuroshio from the Luzon Strait as indicated by surface drifters, which is likely a near-surface phenomenon and only contributes a minor part to the total transport of the SCSwwbc. Several topics related to the SCSwwbc are also discussed.  相似文献   

2016下半年安达曼海潮、余流观测分析     

刘延亮  郭景松  王辉武  薛宇欢  KHOKIATTIWONG Somkiat  于卫东 《海洋学报(英文版)》2018,37(9):13-21

The characteristics of currents and tidal currents in the Andaman Sea(AS) are studied during the second half of2016 using observed data from a moored acoustic Doppler current profiler(ADCP) deployed at 8.6°N, 95.6°E.During the observation period, the mean flow is 5–10 cm/s and largely southward. The root mean square and kinetic energies of the low and high frequency flows, which are divided by a cutoff period of 5 d, are at the same level, indicating their identical importance to the total current. A power spectrum analysis shows that intraseasonal oscillations, a tidal-related semilunar month signal, a semidiurnal tidal signal and periods of 3–4 d are prominent. The barocliny of an eddy kinetic energy is stronger than the mean kinetic energy, both of which are the strongest on the bottom and the weakest at 70 m depth. Residual currents are largely southward(northward) during the summer(winter) monsoon season. Two striking peaks of the southward flow cause the 80 d period of meridional currents. The first peak is part of a large-scale circulation, which enters the AS through the northern channel and exits through the southern channel, and the second peak is part of a local vortex. The 40 d oscillation of the zonal current is forced by geostrophic variations attributed to local and equatorial remote forcing. The tidal current is dominated by semidiurnal constituents, and among these, M2 and N2 are the top two largest major axes. Moreover, astronomical tidal constituents MM and MSF are also significant. Diurnal constituents are weak and shallow water tides are ignorable. The aims are to introduce the new current data observed in the AS and to provide initial insights for the tidal and residual currents in the Andaman Sea.  相似文献   

铜藻(Sargassum horneri)在营养限制胁迫后对NH₄-N的超补偿吸收研究     

李大鹏  马增岭  李慧  丁刚  辛美丽  吴海一  郭文 《海洋与湖沼》2018,49(4):904-909

以经济马尾藻铜藻(Sargassum horneri)为研究材料,研究了其在营养限制胁迫后对NH_4-N的超补偿吸收情况。实验分营养限制和恢复营养两个阶段进行,每个阶段均设置饥饿处理组、饱和处理组和正常对照组。铜藻在低营养限制胁迫(饥饿)下培养10天后,恢复营养盐培养3天,分别采用次溴酸盐氧化法和锌镉还原法测定培养液中的NH_4-N和NO_3-N的浓度。本文研究发现,饥饿处理组中铜藻吸收氨氮的速率远高于正常对照组和饱和处理组,在恢复培养第一天时,饥饿处理组对NH_4-N的吸收速率最高为14.94μmol/(g·h),与正常对照组及饱和处理组间差异显著。在恢复培养的后两天,三个处理组中铜藻对NH_4-N的吸收速率差异慢慢变小,直至最后几乎相同。三组对NO_3-N没有表现出较高的吸收,最高仅为6.15μmol/(g·h),结果表明:氮源包括NH_4-N和NO_3-N时,铜藻优先选择吸收NH_4-N。实验后称重测定生长速率:对照组、饥饿处理组和饱和组生长率(SGR)分别为8.48%、8.86%、8.01%,ANOVA方差分析表明,三者存在显著差异(P=0.0320.05),从而证实了铜藻也存在超补偿生长的现象。  相似文献